February 2025 issue, BioElectRx Business Report
February 28, 2025 | A team of researchers at Washington University in St. Louis is using 3D printing to create bioelectronic scaffolds that would allow researchers to create new tissue with a host of potential applications in bioelectronic medicine. Tissue engineers have used scaffolds in the past to repair neurological and other tissue damage.
In 2004, a team of researchers from the Mayo Clinic reported at the annual meeting of the American Academy of Neurology that a biodegradable implant can act as a scaffold for the regrowth of severed nerves. The implant was seeded with cells and growth factors to encourage nerve regrowth. In 2016, investigators at Rutgers and Stanford reported in Nature Communications that their 3D micro-scaffold technology promotes reprogramming of stem cells into neurons, and supports growth of neuronal connections capable of transmitting electrical signals. The scaffolds, loaded with healthy, beneficial neurons that can replace diseased cells, were injected into mouse brains.
In 2020, a team led by Karen Moxon at UC Davis received a $36.5 million DARPA award to help rebuild pathways between brain and spinal cord in individuals with spinal cord injury. They used stabilized hemodynamics to optimize delivery of neural stem cells using personalized 3D printed scaffolds within two weeks of injury to regenerate lost connections. And in 2023, a team from the University of Hong Kong reported on their use of a microscale scaffold to synthesize conductive hydrogels for bioelectronic implants.
The Washington University team sees another potential application for 3D-printed scaffolding in drug development. “We have many projects in the lab that are envisioned for tissues-on-chips technology, where this could be used for drug development purposes, toxicology, environmental toxicity, and other projects where human tissues are in a dish,” said Alexandra Rutz, an assistant professor of biomedical engineering at the McKelvey School of Engineering. She and Somtochukwu Okafor, a doctoral student in Rutz’s lab, have 3D printed bioelectronic scaffolds that have the properties cells need to form new tissue. They published the result of their work this month in the journal Advanced Materials Technologies.
“Most traditional scaffolds have been on materials that have been inspired by nature, but here, we are taking that concept and applying it to what we call functional materials,” Rutz said. “These are materials that are designed to perform a certain function, such as being electronically conducting.”
The bioelectronic scaffolds that Rutz and Okafor print appear as dark-colored dots about 6 mm in diameter and are floating in water. Okafor has carefully created these tiny scaffolds with a polymer known as PEDOT:PSS, which she has processed into a water-based gel used as an ink.
“When you think of electronics, you aren’t thinking of them being in water,” Okafor said. “But that’s an advantage of using PEDOT:PSS as the conducting material. We are creating hydrated electronics that maintain their electronic properties in an environment that can also sustain living systems.”
Okafor, a fifth-year doctoral student in biomedical engineering, said that while their team isn’t the only one using this process, their work is unique. “Bioelectronics as a field isn’t new—think cochlear implants, pacemakers, and smartwatches—but we are trying to interface with biology to make the technology more like systems that would occur in nature,” Okafor said. “We have borrowed approaches in 3D printing and tissue engineering and combined it with bioelectronics. All these processes have individual advantages that they bring to the table, and we are combining them into one.”
Most traditional materials that conduct electricity are stiff, which may negatively impact formation of tissue. Rutz’s lab created their scaffolds from a soft, conducting hydrogel and with pores that are about 150-300 microns in size that can influence how cells behave within the scaffold, such as how they attach to others, how they move through the scaffold and how they multiply. The pores help to form a lattice-like structure that grows upward to support the cells. The researchers can also adjust the angle of the pores so that the grid lines are diagonal instead of vertical and horizontal. “The pores have to be big enough to allow the cells to move around, but small enough that the cells have structure to hold on to and don’t fall through,” Okafor said.
The tissue the team seeks to create could be used as human tissue or plant tissue, making it versatile for several uses.
